Protein phosphatase 1 regulatory subunit 14A

NameProtein phosphatase 1 regulatory subunit 14A
Synonyms
  • 17 kDa PKC-potentiated inhibitory protein of PP1
  • CPI-17
  • CPI17
  • PPP1INL
  • Protein kinase C-potentiated inhibitor protein of 17 kDa
Gene NamePPP1R14A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009118|Protein phosphatase 1 regulatory subunit 14A
MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRL
EELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHR
QPGLRQPSPSHDGSLSPLQDRARTAHP
Number of residues147
Molecular Weight16692.84
Theoretical pINot Available
GO Classification
Functions
  • protein phosphatase inhibitor activity
Processes
  • small GTPase mediated signal transduction
  • regulation of phosphorylation
Components
  • cytosol
General FunctionProtein phosphatase inhibitor activity
Specific FunctionInhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ96A00
UniProtKB Entry NamePP14A_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0017694|Protein phosphatase 1 regulatory subunit 14A (PPP1R14A)
ATGGCAGCTCAGCGGCTGGGCAAGCGCGTGCTGAGCAAGCTGCAGTCTCCATCGCGGGCC
CGCGGGCCAGGGGGCAGTCCCGGGGGGCTGCAGAAGCGGCACGCGCGCGTCACCGTCAAG
TATGACCGGCGGGAGCTGCAGCGGCGGCTGGACGTGGAGAAGTGGATCGACGGGCGCCTG
GAGGAGCTGTACCGCGGCATGGGACTCCTGAAGTCATGTGGGAAACCTGTCGAGGACTTC
ATCCAGGAGCTGCTGGCAAAGCTTCAAGGCCTCCACAGGCAGCCCGGCCTCCGCCAGCCA
AGCCCCTCCCACGACGGCAGCCTCAGCCCCCTCCAGGACCGGGCCCGGACTGCTCACCCC
TGA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:14871
Chromosome Location19
LocusNot Available
References
  1. Yamawaki K, Ito M, Machida H, Moriki N, Okamoto R, Isaka N, Shimpo H, Kohda A, Okumura K, Hartshorne DJ, Nakano T: Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase. Biochem Biophys Res Commun. 2001 Jul 27;285(4):1040-5. 11467857
  2. Liu QR, Zhang PW, Lin Z, Li QF, Woods AS, Troncoso J, Uhl GR: GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA. Biochem J. 2004 Jan 1;377(Pt 1):171-81. 12974676
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  4. Zemlickova E, Johannes FJ, Aitken A, Dubois T: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47. 15003508
  5. Zahedi RP, Lewandrowski U, Wiesner J, Wortelkamp S, Moebius J, Schutz C, Walter U, Gambaryan S, Sickmann A: Phosphoproteome of resting human platelets. J Proteome Res. 2008 Feb;7(2):526-34. Epub 2007 Dec 19. 18088087
  6. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. 21269460
  7. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. 21406692
  8. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569