mRNA-capping enzyme

NamemRNA-capping enzyme
Synonyms
  • CAP1A
  • HCAP1
  • HCE
Gene NameRNGTT
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009361|mRNA-capping enzyme
MAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVK
MGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPE
LIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDI
EEAPPPPLLPDWCFEDDEDEDEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKG
VTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRY
MMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYL
IYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICT
SRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEG
LLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYN
TAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPRPLT
Number of residues597
Molecular Weight68556.21
Theoretical pINot Available
GO Classification
Functions
  • protein tyrosine phosphatase activity
  • triphosphatase activity
  • RNA guanylyltransferase activity
  • mRNA guanylyltransferase activity
  • protein tyrosine/serine/threonine phosphatase activity
  • polynucleotide 5'-phosphatase activity
  • GTP binding
Processes
  • RNA processing
  • transcription from RNA polymerase II promoter
  • gene expression
  • viral process
  • 7-methylguanosine mRNA capping
  • polynucleotide 5' dephosphorylation
Components
  • nucleus
  • nucleoplasm
General FunctionTriphosphatase activity
Specific FunctionBifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDO60942
UniProtKB Entry NameMCE1_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017442|mRNA-capping enzyme (RNGTT)
ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTG
GCAGGAAGATTCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCT
GAAGAAAATCGGTTCCATCCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAA
ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAA
AAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACT
GAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAA
CTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT
TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGA
CCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATA
GAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA
GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAA
AGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT
GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC
TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT
ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC
ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT
CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT
CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG
ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT
CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC
ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT
TCAAGAAAGAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAGCCTCCCAGT
CTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGGTTACTTCCT
CAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAAATCAAGGTG
ACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAGAACAACAGC
TGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAACACTGCCATG
GCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAGTTCATCGAC
AGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCTGACACGGAG
CTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:10073
Chromosome Location6
LocusNot Available
References
  1. Yue Z, Maldonado E, Pillutla R, Cho H, Reinberg D, Shatkin AJ: Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):12898-903. 9371772
  2. Yamada-Okabe T, Doi R, Shimmi O, Arisawa M, Yamada-Okabe H: Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme. Nucleic Acids Res. 1998 Apr 1;26(7):1700-6. 9512541
  3. Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K: Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme. Biochem Biophys Res Commun. 1998 Feb 4;243(1):101-8. 9473487
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  6. Pillutla RC, Yue Z, Maldonado E, Shatkin AJ: Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. J Biol Chem. 1998 Aug 21;273(34):21443-6. 9705270
  7. Wen Y, Shatkin AJ: Transcription elongation factor hSPT5 stimulates mRNA capping. Genes Dev. 1999 Jul 15;13(14):1774-9. 10421630
  8. Chiu YL, Coronel E, Ho CK, Shuman S, Rana TM: HIV-1 Tat protein interacts with mammalian capping enzyme and stimulates capping of TAR RNA. J Biol Chem. 2001 Apr 20;276(16):12959-66. Epub 2001 Jan 18. 11278368
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  10. Chu C, Das K, Tyminski JR, Bauman JD, Guan R, Qiu W, Montelione GT, Arnold E, Shatkin AJ: Structure of the guanylyltransferase domain of human mRNA capping enzyme. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10104-8. doi: 10.1073/pnas.1106610108. Epub 2011 Jun 2. 21636784