DNA-directed RNA polymerase I subunit RPA34

NameDNA-directed RNA polymerase I subunit RPA34
Synonyms
  • A34.5
  • Antisense to ERCC-1 protein
  • ASE-1
  • ASE1
  • CAST
  • CD3-epsilon-associated protein
  • CD3E-associated protein
  • PAF49
  • RNA polymerase I-associated factor PAF49
Gene NameCD3EAP
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009194|DNA-directed RNA polymerase I subunit RPA34
MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRH
VPLSGSQIVKGKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEG
PQQSLSGSPLQPIPASPPPQIPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQ
VTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLE
PLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLEDTVLSPTKKRKRQKGTEGME
PEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPVEPEMKPLESP
GGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTS
TKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEE
MPGPPLNSESGEEAPTGRDKKRKQQQQQPV
Number of residues510
Molecular Weight54985.335
Theoretical pINot Available
GO Classification
Functions
  • poly(A) RNA binding
  • DNA-directed RNA polymerase activity
Processes
  • negative regulation of gene expression, epigenetic
  • regulation of gene expression, epigenetic
  • rRNA transcription
  • termination of RNA polymerase I transcription
  • transcription elongation from RNA polymerase I promoter
  • transcription from RNA polymerase I promoter
  • transcription initiation from RNA polymerase I promoter
  • transmembrane receptor protein tyrosine kinase signaling pathway
  • gene expression
Components
  • nucleolus
  • nucleoplasm
  • DNA-directed RNA polymerase I complex
  • cytoplasm
  • mitochondrion
  • RNA polymerase I transcription factor complex
  • nucleus
  • chromosome
General FunctionPoly(a) rna binding
Specific FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDO15446
UniProtKB Entry NameRPA34_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017715|DNA-directed RNA polymerase I subunit RPA34 (CD3EAP)
ATGGAGGAGCCCCAGGCCGGCGGTGAGGATGCTGCTCGGTTCTCTTGTCCCCCCAACTTT
ACCGCGAAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGTCCA
GATACGGAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAATGGG
CGGCATGTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGGCAC
CGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCCTCA
ACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATCCTT
GAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCCCCA
CCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCAGTC
ACAGGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAGGAG
ATGCAGGTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTGGAG
GTGGACATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAAAAT
CAGCAGCTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAGACA
CTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAAGGG
AAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAGCCT
CTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAAGGG
ATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCACTG
GAGGAAGCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATGGCA
ATGATGGAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTGGAG
TCCCCAGGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAGGCA
GCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTGGAG
CCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCTCCC
ACATCCACCAAGAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACTGAGCCAATT
CAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCGGGA
TCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCCCAA
GAGGAGATGCCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGCCGG
GACAAGAAGCGGAAGCAGCAGCAGCAGCAGCCTGTGTAG
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:24219
Chromosome Location19
LocusNot Available
References
  1. Whitehead CM, Winkfein RJ, Fritzler MJ, Rattner JB: ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes. Chromosoma. 1997 Dec;106(8):493-502. 9426281
  2. Yamazaki T, Hamano Y, Tashiro H, Itoh K, Nakano H, Miyatake S, Saito T: CAST, a novel CD3epsilon-binding protein transducing activation signal for interleukin-2 production in T cells. J Biol Chem. 1999 Jun 25;274(26):18173-80. 10373416
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  4. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. 17081983
  5. Panov KI, Panova TB, Gadal O, Nishiyama K, Saito T, Russell J, Zomerdijk JC: RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. Mol Cell Biol. 2006 Jul;26(14):5436-48. 16809778
  6. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007 May 25;316(5828):1160-6. 17525332
  7. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. 18669648
  8. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. 19413330
  9. Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. 19690332
  10. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. 20068231
  11. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. 21406692
  12. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18. 22814378
  13. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569
  14. Hendriks IA, D'Souza RC, Yang B, Verlaan-de Vries M, Mann M, Vertegaal AC: Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol. 2014 Oct;21(10):927-36. doi: 10.1038/nsmb.2890. Epub 2014 Sep 14. 25218447